Woodford Investment Management raised the amount during the offer period, which began on 2 June and closed on 19 June.
Daily pricing of the fund started on 20 June.
Since trading began at noon on 19 June 2014, about 95% of the portfolio has been invested in the market.
Craig Newman, chief executive of Woodford Investment Management, said: "We are thrilled to have had such support from investors.
"We understand the great responsibility we have been given to deliver the long-term investment returns they anticipate and we are committed to achieving that objective."
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Woodford Investment Management intends to disclose the entire portfolio on a monthly basis on its website, starting in July.
Mr Newman said: "We want to be open and transparent with our investors and this is why we will publish the fund's entire portfolio on the website so that everyone can see where their money is invested, rather than just the top ten holdings as many companies do.
"We expect this to be available by mid-July."
Neil Woodford, head of investment, Woodford Investment Management, said: "I am really excited with the portfolio that I have been able to build for this fund.
"I have met businesses in recent weeks that have immense potential and offer fantastic investment value. My entire focus is finding companies like this that I believe can deliver long-term value to the investors that have the confidence to invest in my fund."
The CF Woodford Equity Income Fund aims to provide investors with long-term appreciation by investing in stocks primarily listed on the UK stock exchanges, with up to 20% invested in international companies, delivering a yield 10% higher than the FTSE All Share Index yield.