Chief operating officer Nick Hamilton and chief legal and compliance officer Gray Smith will leave the company, which started up this year following Neil Woodford's departure from Invesco Perpetual.
Craig Newman, chief executive of Woodford Investment Management, said: "Having assisted with the successful set up and launch of Woodford Investment Management LLP in their respective roles of chief operating officer and chief legal & compliance officer, Nick Hamilton and Gray Smith will be leaving the firm this month.
"Nick and Gray helped us get over the line. We thank them for their commitment and wish them all the best in the future."
The company, which has over 30 staff on its books, has more than £8.5bn of assets under management and advice.
{desktop}{/desktop}{mobile}{/mobile}

It has recently appointed a number of senior managers to strengthen its compliance and operations team. They include the dual appointment of Simon Osborne as head of compliance and Gavin St. John-Heath as chief risk and operations officer.
Simon Osborne joined from Los Angeles-based Nuveen Investments where he worked since 2010, as senior vice president. Prior to this, Simon gained eight years' experience managing teams in compliance divisions at PIMCO, in London and at its Newport Beach HQ.
Gavin St. John-Heath has more than eight years' experience having held several senior risk management and compliance roles across various market sectors with a number of leading companies, including Petronas Energy Trading. He holds a D.Phil. in computational neuroscience and will join from BlueQuant Capital Management on 1 January 2015.
Two others have also joined, with David Brailsford coming in as data architecture delivery manager from IBM and Vimal Patel, business analyst, arriving from Abu-Dhabi-based sovereign wealth manager Mubadala Development Company, where he was head of investment systems.
Mr Newman said: "As our business continues to grow, the demands placed on our legal and compliance operation will intensify. We have brought in experienced specialists to help implement procedures, which will cement our goal of building a fund management company that has the cutting-edge operational platform needed in a regulation-heavy, post-financial crisis world."

Get FREE daily news summaries direct to your inbox. Sign up on the homepage now.

Follow us on Twitter  and get frequent news alerts @FPM_online.

Or follow Editor Kevin O'Donnell - @FPM_Kevin or staff writer James Nadal - @FPM_James.

For the latest Sipp, SSAS and retirement news visit our sister news site www.sippsprofessional.co.uk and on Twitter @SippsPro.